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Liquid Chromatography Lab Kool Aid Answers: Separation of FD&C Dyes
"The liquid chromatography lab for Kool-Aid involves separating food dyes, typically Red 40 and Blue 1, using a C18 reverse-phase stationary phase. By passing increasing concentrations of isopropyl alcohol through a Sep-Pak cartridge, you selectively elute dyes based on their polarity. Red 40 elutes first with lower alcohol concentrations, while Blue 1 elutes later as the mobile phase becomes less polar. Understanding the Principles of Liquid Chromatography Liquid chromatography is a fundamental analytical technique used to separate a mixture into its individual components. In the context of the Kool-Aid lab, the primary objective is to separa..."
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